Describe a common pitfall when performing the Gram stain on an older culture.

Describe a common pitfall when performing the Gram stain on an older culture.

• A. Older cells decolorize more easily, causing Gram-positive organisms to appear Gram-negative or weakly staining.
• B. Older cells always stain Gram-positive.
• C. Older cells stain more strongly.
• D. Older cells may decolorize more easily, causing Gram-positive organisms to appear Gram-negative or weakly staining.

Answer: (D)