A disk diffusion method used to assess antibiotic susceptibility is called the

A disk diffusion method used to assess antibiotic susceptibility is called the

• A. Bacteriostatic
• B. Bactericidal
• C. Kirby-Bauer Test
• D. Minimum Inhibitory Concentration (MIC)

Answer: (C)

The main idea here is the standardized disk diffusion method used to test antibiotic susceptibility. In this approach, a bacterial lawn is spread on a Mueller–Hinton agar plate, and paper disks impregnated with specific antibiotics are placed on the surface. After incubation, the antibiotics diffuse into the agar and inhibit growth around the disks, producing clear zones whose diameters are measured. The Kirby-Bauer test is the standardized version of this disk diffusion method, using fixed inoculum density, agar depth, and disk concentrations so the results can be interpreted consistently with established breakpoints. The other terms describe different aspects or methods: bacteriostatic and bactericidal refer to whether an antibiotic merely inhibits growth or kills bacteria, not the testing method itself; and minimum inhibitory concentration is the lowest concentration that prevents growth, typically determined by dilution methods rather than disk diffusion.

The main idea here is the standardized disk diffusion method used to test antibiotic susceptibility. In this approach, a bacterial lawn is spread on a Mueller–Hinton agar plate, and paper disks impregnated with specific antibiotics are placed on the surface. After incubation, the antibiotics diffuse into the agar and inhibit growth around the disks, producing clear zones whose diameters are measured. The Kirby-Bauer test is the standardized version of this disk diffusion method, using fixed inoculum density, agar depth, and disk concentrations so the results can be interpreted consistently with established breakpoints.

The other terms describe different aspects or methods: bacteriostatic and bactericidal refer to whether an antibiotic merely inhibits growth or kills bacteria, not the testing method itself; and minimum inhibitory concentration is the lowest concentration that prevents growth, typically determined by dilution methods rather than disk diffusion.